Document Type : Original Article
Authors
1 University of Mazandaran
2 Tabriz University of Medical Sciences
Abstract
This study explores the induction of apoptosis in colon cancer stem cells (CCSCs) within a three-dimensional culture milieu, utilizing the innovative cold atmospheric plasma (CAP) transfer technique known as TCAP. TCAP, a gas mixture partially or fully ionized, comprises photons, charged and neutral particles, and free radicals and has garnered attention in biomedical realms such as cancer therapy. TCAP exerts its influence on CCSCs via a continuous transport mechanism, facilitating the effective conveyance of reactive oxygen and nitrogen species (RONS). Key cellular determinants of CCSCs influenced by TCAP treatment, encompassing the count of apoptotic cells and the expression of Bax and Bcl-2 proteins, were assessed employing Annexin V and qrt-ELISA methodologies. The outcomes of TCAP treatment on CCSCs manifest a notable escalation in apoptotic cell count, coupled with favorable alterations in the expression of Bcl-2 and Bax genes. Furthermore, this investigation underscores the anticancer efficacy of TCAP and its potency in diminishing the survival rate of CCSCs. Nonetheless, additional preclinical and clinical trials are imperative to comprehensively assess the efficacy, safety, and potential synergistic interactions of TCAP with adjunctive therapeutic modalities. In sum, TCAP presents a promising alternative for combating CCSCs.
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